AKURASI ALAT DETEKSI SODIUM DALAM URIN SECARA OTOMATIS DENGAN METODE ELECTRODA SELEKSI ION KERING

Basuki Budiman, Kartika Pela, Aya Yuriesta Arifin, Ferbriani Ferbriani, Amalia Safitri, Dwi Anggraini, Rinda Ayu Dewi, Athena Anwar

Abstract


Inductively Couple Plasma (ICP) is a standard method to detect urinary sodium. However, the urinary sodium analysis method has a limitation in which the process to free sodium from molecules is not digest thoroughly. Ionic Selected Electrode (ISE) method is the better option. A New authomatic instrument Na-K-Cl automatic digital analyser that is “Spotchem EL 1520” using ISE method (dry ISE) was developed but the application to detect urinary sodium has not yet done. The purpose of this trial was to test the accuracy of the instrument in detecting urinary sodium. A number of 100 people  aged 20-64 years participated in the trial by  collecting around 50 mL fresh urine. Urinary sodium was analysed by ICP method in chemical laboratory and by ISE method using the digital analyser instrument. A number of 6 out of 100 urine sample did not meet a minimum volume requirement to be analysed by ICP method but it was able to be analysed using ISE method. The instrument was able to detect urinary sodium more than 132 (SD:77,81) mmol/L compared to Laboratory ICP method 79.38 (SD: 47,50) mmol/L. The Sensitivity of the instrument to detect sodium in urine was 97.6% (95% CI : 87.1-99.9%) and the Specivicity was 58 % (95% CI: 44.7-71.9). The trial also analysed urinary creatinine with aution instrument. A number of 10 urine sample was analysed by private clinical laboratory as quality control.  Aution is semi quantitave digital analyser and the result was inline with quantitative (controlled sample). The automatic digital analyser can be used as alternative for conventional analyses of urinary sodium.

ABSTRAK

Metode deteksi konsentrasi sodium dapat dilakukan dengan metode ICP atau ISE.  Saat ini telah dikembangkan alat deteksi sodium metode ISE kering yang dapat diterapkan pada sampel serum maupun urin. Uji coba alat dilakukan di laboratorium terpadu Badan Litbang Kesehatan di Bogor. Sebanyak 100 orang partisipan terlibat dalam uji coba. Partisipan  adalah penduduk di sekitar kantor dan honorer penelitian kohor tumbuh kembang di Bogor. Partisipan diminta specimen urin di laboratorium dan diperiksa kadarnya pada hari itu juga. Dua alat yang diuji coba adalah Na-K-Cl digital analyzer, (Spotchem EL 1520, arkray dengan metode ISE kering) dan creatinine aution, semi kuantitatif (arkray). Keduanya menggunakan urin sebagai specimen. Uji coba dimulai dengan pemeriksaan deteksi kreatinin karena pemeriksaan harus kurang dari 24 jam. Sebanyak 50 sampel diperiksa berurutan tanpa jeda, kemudian diteruskan 50 spesimen lainnya. Kesepakatan supervisor, diperiksakan 10 specimen diperiksa di laboratorium swasta yang ada di Bogor sebagai pembanding. Ujicoba alat Na-K-Cl analyzer dengan 100 specimen urin dilakukan  tanpa jeda. Pembanding hasil periksa alat ini adalah pemeriksaan urin metode ICP di laboratorium terpadu dan dikerjakan oleh analis kimia yang berpengalaman. Dari segi proses, kedua alat tidak ditemukan masalah saat ujicoba. Kedua alat ini digunakan untuk memeriksa 25 spesimen per hari. Hasil kadar kreatinin setelah dibandingkan dengan hasil periksa di laboratorium swasta secara grafik bagus walaupun dengan nilai absolut berbeda. Uji statistik menunjukkan Se 97,6 dan Sp 58. Hasil deteksi dengan alat ini lebih sensitive dibandingkan dengan laboratorium.

Kata kunci: Inductively Couple Plasma ( ICP), Ionic Selected Electrode (ISE), sodium urin




Keywords


Inductively Couple Plasma ( ICP), Ionic Selected Electrode (ISE), urinary sodium

References


Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar Tahun 2013. Badan Litbang Kesehatan 2014

Cappuccio FP, Simon Capewell S, Lincoln P chief, Klim McPherson K. Policy options to reduce population salt intake. BMJ 2011;343. crossref

He FJ, Campbell NRC, MacGregor GA. Reducing salt intake to prevent hypertension and cardiovasculardisease. Rev Panam Salud Publica. 2012;32(4):293–300.

Hawkes C, Webster J.National Approaches to Monitoring Population Salt Intake: A Trade-Off between Accuracy and Practicality? PLoS ONE 2012;7(10):e46727. crossref

Campbell NRC, Dary O, Cappuccio FP, Neufeld LM, Harding KB, Zimmermann MB. Need for coordinated programs to improve global health by optimizing salt and iodine intake. Rev Panam Salud Publica. 2012;32(4):281–6

MedlinPlus. Sodium urin test. US National Library of Medicine, National Institute of Health. URL://www.nlm.nih.gov/medlineplus/ency/article/003599.htmdiakses 20 Juni 2016

O`Donnel M, Mente A, Yusuf S. Sodium Intake and Cardiovascular Health. CircRest2015; 116; 1046-1057. crossref

Samsudin M, Nurcahyani YD, Ihsan N. Dampak intervensi garam beriodium berbagai dosis terhadap status iodium dan fungsi tiroid normal pada anak sekolah dasar. Media Gizi Mikro Indonesia 2016;8(1):1-16

WHO/PAHO. Regional expert group for cardiovascular disease prevention through population wide dietary salt reduction. Subgroup for research and surveillance. Protocol for population level Sodium determination in 24 hour urine sample. Washington DC. WHO/PAHO 2010

WHO. Guideline: Sodium intake for adults and children. Geneva, World Health Organization (WHO), 2012

Day NE, McKeown N, Wong MY, Welch A, and Binghan S. Epidemiological assessment of diet: a comparison of a 7-day dairy with a food frequency using urinary markers of nitrogen, potassium and sodium. Intl J Epid 2001;30:309-317

Anonious-Hidrolology Project. How to measure sodium. Training Module number WQ-44. World Bank and The Netherlands. New Delhi, India 2000.

Intersalt Cooperative Research Group. Intersalt: an international study of electrolyte excretion and blood pressure. Results for 24 hour urinary sodium and potassium excretion BMJ 1988;297:319-328


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